Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB2872-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Erythropoietin/EPO Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Erythropoietin/EPO in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
CAA26094
- Isotype
- IgG
- Antibody clone number
- 971019
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Human Erythropoietin/EPO Antibody. Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (Catalog # 287-TC) stimulates proliferation in theTF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB2872). The ND50 is typically 0.02-0.2 µg/mL.