- PA5-22261 - Invitrogen Antibodies GNAS antibody

Submitted references Combined Small Cell Carcinoma of the Lung: Is It a Single Entity?

Zhao X, McCutcheon JN, Kallakury B, Chahine JJ, Pratt D, Raffeld M, Chen Y, Wang C, Giaccone G
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2018 Feb;13(2):237-245

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of XLalphas using A) 30 µg NIH-3T3 whole cell lysate and B) 30 µg JC whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a XLalphas polyclonal antibody (Product # PA5-22261) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of XLalphas using 50 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a XLalphas polyclonal antibody (Product # PA5-22261) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of XLalphas using 50 µg rat brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a XLalphas polyclonal antibody (Product # PA5-22261) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of XLalphas using 30 µg of A) HepG2 and B) Raji lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a XLalphas polyclonal antibody (Product # PA5-22261) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot using GNAS Polyclonal Antibody (Product # PA5-22261). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAS Polyclonal Antibody (Product # PA5-22261) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of GNAS was achieved by transfecting Hep G2 with GNAS specific siRNAs (Silencer® select Product # s526399, s526398). Western blot analysis (Fig. a) was performed using whole cell extracts from the GNAS knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with GNAS Polyclonal Antibody (Product # PA5-22261, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to GNAS.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot was performed using Anti-GNAS Polyclonal Antibody (Product # PA5-22261) and a 50 kDa band corresponding to GNAS was observed in Jurkat, ACHN, MCF7, OVCAR3, Hep G2, Mouse Brain and Mouse Adipose, but not in A549, MDA-MB-231 and Rat Brain. An uncharacterized band was observed at ~52 kDa. Whole cell lysates (30 µg lysate) of Jurkat (Lane 1), ACHN (Lane 2), A549 (Lane 3), MCF7 (Lane 4), MDA-MB-231 (Lane 5), OVCAR-3 (Lane 6), Hep G2 (Lane 7), tissue Lysates (30 µg lysate) of Mouse Brain (Lane 8), Mouse Adipose (Lane 9) and Rat Brain (Lane 10) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using GNAS (Product # PA5-22261) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-22261

Antibody data