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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA319134 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-Jagged 1 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-Jagged 1 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- JAG1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 500 µg
- Concentration
- 1.0 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5).? Lane 4 contained sample buffer only.Each lane contains approximately 20 ug of lysate.? Primary antibody was used at a 1:500 dilution. Predicted molecular weight is 134 kDa.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence microscopy using Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells.? Primary antibody was used at a 1:500 dilution. The Jagged1 (green staining) is local-ized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue).? Personal Communication.? Aihua Ma, Univdersity of Cardiff.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human cervical cancer tissue (40X magnification) using Protein A purified anti-Jagged-1 antibody. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:100 dilution of primary antibody was used. Personal Communication. Martin Kast Laboratory.
- Validation comment
- IHC
