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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-485-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IFN-gamma Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human IFN-gamma , recombinant cotton rat IFN-gamma , recombinant feline IFN-gamma , recombinant rat IFN-gamma , recombinant porcine IFN-gamma , and recombinant canine IFN-gamma is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The JAK inhibitor tofacitinib ameliorates immune‑mediated liver injury in mice.
IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
Interferon gamma-dependent intestinal pathology contributes to the lethality in bacterial superantigen-induced toxic shock syndrome.
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
Wang H, Feng X, Han P, Lei Y, Xia Y, Tian D, Yan W
Molecular medicine reports 2019 Dec;20(6):4883-4892
Molecular medicine reports 2019 Dec;20(6):4883-4892
IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS
The Journal of clinical investigation 2011 Oct;121(10):3846-59
The Journal of clinical investigation 2011 Oct;121(10):3846-59
Interferon gamma-dependent intestinal pathology contributes to the lethality in bacterial superantigen-induced toxic shock syndrome.
Tilahun AY, Holz M, Wu TT, David CS, Rajagopalan G
PloS one 2011 Feb 3;6(2):e16764
PloS one 2011 Feb 3;6(2):e16764
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA Jr, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 7;106(14):5954-9
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 7;106(14):5954-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN-gamma Antibody. Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-485-NA). The ND50 is typically 0.2-1.0 µg/mL.
