Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- IC485P-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal IFN-gamma Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IFN-gamma in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) IFN-gamma , rrIFN-gamma , rpIFN-gamma , rrmIFN-gamma , rfeIFN-gamma , or rcaIFN-gamma .
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of IFN-gamma in Mouse Splenocytes Stimulated to Induce Th1 cells by Flow Cytometry. Mouse splenocytes either (A) stimulated to induce Th1 cells or (B) unstimulated were stained with Rat Anti-Mouse IFN-gamma PE-conjugated Monoclonal Antibody (Catalog # IC485P) and Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
