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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC485N-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal IFN-gamma Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IFN-gamma in Western blots. In Western blots, this antibody does not cross-react with recombinant human IFN-gamma , recombinant rat IFN-gamma , recombinant porcine IFN-gamma , recombinant rhesus macaque IFN-gamma , recombinant feline IFN-gamma , or recombinant canine IFN-gamma .
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Near infrared dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of IFN-gamma in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) stimulated to induce Th1 cells or (B) unstimulated were stained with Rat Anti-Mouse IFN-gamma Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC485N) and Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P). Quadrant markers were set based on control antibody staining (Catalog # IC006N). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
