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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF565 - Provider product page
- Provider
- R&D Systems
- Product name
- Cotton Rat IFN-gamma Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects cotton rat IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 0.25% cross-reactivity with recombinant canine IFN-gamma , recombinant feline IFN-gamma , and recombinant human IFN-gamma , recombinant mouse IFN-gamma , recombinant rat IFN-gamma , and recombinant porcine IFN-gamma is observed.
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9QXX2- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Immunization with Live Human Rhinovirus (HRV) 16 Induces Protection in Cotton Rats against HRV14 Infection.
Development of Acquired Immunity following Repeated Respiratory Syncytial Virus Infections in Cotton Rats.
Role of AKT kinase in measles virus replication.
Sendai virus recombinant vaccine expressing hPIV-3 HN or F elicits protective immunity and combines with a second recombinant to prevent hPIV-1, hPIV-3 and RSV infections.
Respiratory syncytial virus (RSV) fusion protein expressed by recombinant Sendai virus elicits B-cell and T-cell responses in cotton rats and confers protection against RSV subtypes A and B.
Developmental pattern of expression of BMP receptors and Smads and activation of Smad1 and Smad5 by BMP9 in mouse basal forebrain.
Patel MC, Pletneva LM, Boukhvalova MS, Vogel SN, Kajon AE, Blanco JCG
Frontiers in microbiology 2017;8:1646
Frontiers in microbiology 2017;8:1646
Development of Acquired Immunity following Repeated Respiratory Syncytial Virus Infections in Cotton Rats.
Yamaji Y, Yasui Y, Nakayama T
PloS one 2016;11(5):e0155777
PloS one 2016;11(5):e0155777
Role of AKT kinase in measles virus replication.
Carsillo M, Kim D, Niewiesk S
Journal of virology 2010 Feb;84(4):2180-3
Journal of virology 2010 Feb;84(4):2180-3
Sendai virus recombinant vaccine expressing hPIV-3 HN or F elicits protective immunity and combines with a second recombinant to prevent hPIV-1, hPIV-3 and RSV infections.
Zhan X, Slobod KS, Krishnamurthy S, Luque LE, Takimoto T, Jones B, Surman S, Russell CJ, Portner A, Hurwitz JL
Vaccine 2008 Jun 25;26(27-28):3480-8
Vaccine 2008 Jun 25;26(27-28):3480-8
Respiratory syncytial virus (RSV) fusion protein expressed by recombinant Sendai virus elicits B-cell and T-cell responses in cotton rats and confers protection against RSV subtypes A and B.
Zhan X, Hurwitz JL, Krishnamurthy S, Takimoto T, Boyd K, Scroggs RA, Surman S, Portner A, Slobod KS
Vaccine 2007 Dec 17;25(52):8782-93
Vaccine 2007 Dec 17;25(52):8782-93
Developmental pattern of expression of BMP receptors and Smads and activation of Smad1 and Smad5 by BMP9 in mouse basal forebrain.
Lopez-Coviella I, Mellott TM, Kovacheva VP, Berse B, Slack BE, Zemelko V, Schnitzler A, Blusztajn JK
Brain research 2006 May 9;1088(1):49-56
Brain research 2006 May 9;1088(1):49-56
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IFN-gamma Inhibition of EMC-induced Cytopathy and Neutralization by Cotton Rat IFN-gamma Antibody. Recombinant Cotton Rat IFN-gamma (Catalog # 565-RG) reduces the encephalomyocarditis (EMC)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMC activity elicited by Recombinant Cotton Rat IFN-gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565). The ND50 is typically 0.3-1.2 µg/mL.
