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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 44-988A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-PKA alpha/beta (Thr197) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- PKA alpha-beta (catalytic domain) (pT197) phosphospecific antibody. Lysates prepared from Y1 Adrenocortical cell lysates untreated (1-4) or treated with l phosphatase (5), then incubated with anti-PKA (pT197) after prior incubation with: no peptide (1, 5), the non-phosphopeptide (2), a generic (pT) peptide (3), or, the phosphopeptide immunogen (4). Data show antibody phospho-site specificity by peptide competition, and that phosphatase stripping eliminates the signal, as expected.
