Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunohistochemistry [5]
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Validation data
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- Product number
- LS-C357511 - Provider product page
- Provider
- LSBio
- Product name
- RAD51 / RECA Antibody (aa1-258) LS-C357511
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Rat
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rad51 antibody Western blot. All lanes: Anti Rad51 at 0.5 ug/ml. Lane 1: 22RV1 Whole Cell Lysate at 40 ug. Lane 2: SW620 Whole Cell Lysate at 40 ug. Lane 3: PANC Whole Cell Lysate at 40 ug. Lane 4: U87 Whole Cell Lysate at 40 ug. Lane 5: CEM Whole Cell Lysate at 40 ug. Lane 6: MM231 Whole Cell Lysate at 40 ug. Predicted band size: 37 kD. Observed band size: 37 kD.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of Rad51 using anti-Rad51 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human HL-60 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Rad51 at approximately 39KD. The expected band size for Rad51 is at 37KD.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of Rad51 using anti-Rad51 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testicular tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testicular tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates, Lane 7: mouse RAW246. 7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Rad51 at approximately 39KD. The expected band size for Rad51 is at 37KD.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rad51 antibody Western blot. All lanes: Anti Rad51 at 0.5 ug/ml. Lane 1: 22RV1 Whole Cell Lysate at 40 ug. Lane 2: SW620 Whole Cell Lysate at 40 ug. Lane 3: PANC Whole Cell Lysate at 40 ug. Lane 4: U87 Whole Cell Lysate at 40 ug. Lane 5: CEM Whole Cell Lysate at 40 ug. Lane 6: MM231 Whole Cell Lysate at 40 ug. Predicted band size: 37 kD. Observed band size: 37 kD.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of Rad51 using anti-Rad51 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human HepG2 whole cell lysates, Lane 8: human HL-60 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Rad51 at approximately 39KD. The expected band size for Rad51 is at 37KD.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of Rad51 using anti-Rad51 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testicular tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testicular tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates, Lane 7: mouse RAW246. 7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Rad51 at approximately 39KD. The expected band size for Rad51 is at 37KD.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rad51 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rad51 antibody IHC-paraffin: Rat Intestine Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rad51 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rad51 antibody IHC-paraffin: Rat Intestine Tissue.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rad51 antibody IHC-paraffin: Human Intestinal Cancer Tissue.