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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NBP2-20606 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal AP-2 beta/TFAP2B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AP-2 beta/TFAP2B Antibody [NBP2-20606] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with AP2 beta antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AP-2 beta/TFAP2B Antibody [NBP2-20606] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: AP-2 beta/TFAP2B Antibody [NBP2-20606] - IP samples: MCF-7 whole cell extract A. 30 ug MCF-7 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of AP2 beta protein by 4 ug AP2 beta antibody 7.5 % SDS-PAGE The mmunoprecipitated AP2 beta protein was detected by AP2 beta antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
