Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 720238 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLUT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
- Concentration
- 0.5 mg/mL
Submitted references The RNA-Binding Protein NONO Coordinates Hepatic Adaptation to Feeding.
Benegiamo G, Mure LS, Erikson G, Le HD, Moriggi E, Brown SA, Panda S
Cell metabolism 2018 Feb 6;27(2):404-418.e7
Cell metabolism 2018 Feb 6;27(2):404-418.e7
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of HepG2 (Lane 1), Caco2 (Lane 2), HEK-293 (Lane 3), NIH/3T3 (Lane 4), C2C12 (Lane 5), PC-12 (Lane 6). The blots were probed with Anti-GLUT2 Rabbit Polyclonal antibody (Product # 720238, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~ 57 kDa band corresponding to GLUT2 was observed in all cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot®2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).