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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Other assay [1]
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- Product number
- PA1-32136 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IkB beta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A control peptide is available, GTX27548.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 100 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Lower threshold to NFκB activity sensitizes murine β-cells to streptozotocin.
Maturation of the Acute Hepatic TLR4/NF-κB Mediated Innate Immune Response Is p65 Dependent in Mice.
CpG-ODN-mediated TLR9 innate immune signalling and calcium dyshomeostasis converge on the NFκB inhibitory protein IκBβ to drive IL1α and IL1β expression.
Wright CJ, McKenna S, De Dios R, Boehmer BH, Nguyen L, Ghosh S, Sandoval J, Rozance PJ
The Journal of endocrinology 2021 May 6;249(3):163-175
The Journal of endocrinology 2021 May 6;249(3):163-175
Maturation of the Acute Hepatic TLR4/NF-κB Mediated Innate Immune Response Is p65 Dependent in Mice.
Zarate MA, Nguyen LM, De Dios RK, Zheng L, Wright CJ
Frontiers in immunology 2020;11:1892
Frontiers in immunology 2020;11:1892
CpG-ODN-mediated TLR9 innate immune signalling and calcium dyshomeostasis converge on the NFκB inhibitory protein IκBβ to drive IL1α and IL1β expression.
De Dios R, Nguyen L, Ghosh S, McKenna S, Wright CJ
Immunology 2020 May;160(1):64-77
Immunology 2020 May;160(1):64-77
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 1 Ontogeny of the NF-kappaB subunits shows that p65 protein is developmentally regulated. Unexposed mouse livers from different developmental time points were collected and measured for hepatic (A) cRela and Nfkbl gene expression, (B,C) whole cell lysate and (D,E) nuclear protein p65 and p50 (F) , whole cell lysate protein p105, and (G) cytoplasmic NF-kappaB inhibitory proteins IkappaBalpha and IkappaBbeta levels. GAPDH and HDAC1 were used as loading controls for the cytosolic and nuclear extracts, respectively. * P < 0.05 vs. adult (AD) by 1-way ANOVA and Dunnet post-hoc test. Values are represented as fold change relative to adult (AD).
