MA5-15673

antibody from Invitrogen Antibodies

Targeting: NFKBIB IKBB, TRIP9

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15673

Provider

Invitrogen Antibodies

Product name

IkB beta Monoclonal Antibody (7B4)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15673 targets NFKBIB in WB applications and shows reactivity with Human samples.

Antibody clone number

7B4

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NFKBIB using NFKBIB monoclonal antibody (Product # MA5-15673) in Jurkat (1) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-IKB beta Antibody (Product # MA5-15673) and a 45 kDa band corresponding to IKKB was observed across the cell lines tested. Faint uncharacterized bands (*) were also observed. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), Jurkat (Lane 2), THP-1 (Lane 3), A549 (Lane 4), HeLa (Lane 5) and Raji (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti- Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NFKBIB using NFKBIB monoclonal antibody (Product # MA5-15673) in Jurkat (1) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of IKB beta was performed using A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IKB beta Mouse Monoclonal Antibody (Product # MA5-15673) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing nuclear and cytoplasmic localization of IKB beta in A549 cells. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.