702556

antibody from Invitrogen Antibodies

Targeting: CDK4 PSK-J3

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

702556

Provider

Invitrogen Antibodies

Product name

Phospho-CDK4 (Thr172) Recombinant Rabbit Monoclonal Antibody (9H2L7)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Sheep, Rat and Mouse.

Antibody clone number

9H2L7

Concentration

0.5 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, T98-G cells were fixed and permeabilized for detection of endogenous Cdk 4 (pT 172) using Cdk 4 (pT 172) Recombinant Rabbit Monoclonal Antibody (Product # 702556, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Detection and localization of CDK4 pT172 (green) in the nucleus can be clearly observed in cell cycle synchronized cells (serum starved overnight and serum released for 10 hours) as compared to untreated cells. Antibody specificity was demonstrated by competition with the CDK4 pT172 phospho peptide, which results in loss of signal. No competition was observed with the non-phospho peptide. The images were captured at 60X magnification.