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- Western blot [1]
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- Product number
- MA5-17247 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-LDHA Monoclonal Antibody (AF14A11)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- A suggested positive control for this product is HeLa cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AF14A11
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Enhanced glycolytic metabolism supports transmigration of brain-infiltrating macrophages in multiple sclerosis.
Kaushik DK, Bhattacharya A, Mirzaei R, Rawji KS, Ahn Y, Rho JM, Yong VW
The Journal of clinical investigation 2019 May 21;129(8):3277-3292
The Journal of clinical investigation 2019 May 21;129(8):3277-3292
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), Hep G2 (Lane 3), HEK-293 (Lane 4), IMR-32 (Lane 5), MDA-MB-231 (Lane 6), A-431 (Lane 7), K-562 (Lane 8) and Jurkat (Lane 9).The blots were probed with LDHA Mouse monoclonal Antibody (Product # MA5-17247, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 37 kDa band corresponding to LDHA was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
