Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
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Validation data
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- Product number
- LS-C744804 - Provider product page
- Provider
- LSBio
- Product name
- LDHA / LDH1 Antibody (Liquid, HRP) LS-C744804
- Antibody type
- Polyclonal
- Description
- Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
- Reactivity
- Rabbit
- Host
- Goat
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of Goat Anti-Lactate Dehydrogenase antibody. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 µg per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4°C. Secondary antibody: secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western Blot of Goat Anti-Lactate Dehydrogenase antibody. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 µg per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4°C. Secondary antibody: secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4°C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western Blot of Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.