LS-C46068

antibody from LSBio

Targeting: LIME1 dJ583P15.4, FLJ20406, LIME

Immunoprecipitation (Enhanced validation)

Antibody Data

Product number

LS-C46068

Provider

LSBio

Product name

LIME1 / LIME Antibody (aa141-295, clone LIME-06) LS-C46068

Antibody type

Monoclonal

Description

Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

LIME-06

Storage

Store at 2°C to 8°C. Do not freeze.

Immunoprecipitation

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92) or anti-human CD4 (MEM-16), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 antibody (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92) or anti-human CD4 (MEM-16), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 antibody (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92) or anti-human CD4 (MEM-16), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 antibody (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).