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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Flow cytometry [1]
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- Product number
- NB100-64392AF488 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Neutrophil Elastase/ELA2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. NB100-64392 is specific for human neutrophil elastase.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (39A) [Alexa Fluor® 488] [NB100-64392AF488] - Leukocytes were isolated from BAL fluid of a CF patient and stained for analysis by flow cytometry. Macrophages were found to have scavenged substantial neutrophil elastase from the airways while minimal was observed on the surface of T cells from the same patient as expected. This antibody works well for staining multiple cell types and obtains reliable fluorescence in the FITC channel. When used for single-stained beads compensation controls, consistent signal is obtained provided that the cytometer is accurately calibrated. One thing to be aware of is that different lots of this antibody have slightly different concentrations. Flow cytometry image submitted by a verified customer review.
