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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC91671R-100UG - Provider product page
- Provider
- R&D Systems
- Product name
- Human Neutrophil Elastase/ELA2 Alexa Fluor® 647-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human ELA2 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Antigen sequence
P08246- Isotype
- IgG
- Antibody clone number
- 950317
- Vial size
- 100 ug
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Neutrophil Elastase/ELA2 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line treated with 3 μM monensin for 3 hours was stained with Mouse Anti-Human Neutrophil Elastase/ELA2 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC91671R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Membrane-associated Proteins.
