PA1-077-A555

antibody from Invitrogen Antibodies

Targeting: GOLGA2 GM130, golgin-95

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-077-A555

Provider

Invitrogen Antibodies

Product name

GM130 Polyclonal Antibody, Alexa Fluor™ 555

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1077A555 detects GM130 from human samples.

Concentration

1 mg/mL

References

Submitted references

Progeny Varicella-Zoster Virus Capsids Exit the Nucleus but Never Undergo Secondary Envelopment during Autophagic Flux Inhibition by Bafilomycin A1.

Girsch JH, Walters K, Jackson W, Grose C
Journal of virology 2019 Sep 1;93(17)

---

A MST1-FOXO1 cascade establishes endothelial tip cell polarity and facilitates sprouting angiogenesis.

Kim YH, Choi J, Yang MJ, Hong SP, Lee CK, Kubota Y, Lim DS, Koh GY
Nature communications 2019 Feb 19;10(1):838

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GM-130 (orange) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a GM-130 Polyclonal Antibody, AlexaFluor 555 conjugate (Product # PA1-077-A555) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.