- PA5-23509 - Invitrogen Antibodies GBP1 antibody

Submitted references Genetic Regulation of Guanylate-Binding Proteins 2b and 5 during Leishmaniasis in Mice.

Sohrabi Y, Volkova V, Kobets T, Havelková H, Krayem I, Slapničková M, Demant P, Lipoldová M
Frontiers in immunology 2018;9:130

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of GBP1 in human HCT-116 lysate in the absence (1) and presence (2) of immunizing peptide and mouse RAW cell lysate (3). Samples were incubated in GBP1 polyclonal antibody (Product # PA5-23509) using a dilution of 3 µg/mL followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of GBP1 in Human rectum tissue. Samples were incubated in GBP1 polyclonal antibody (Product # PA5-23509) using a dilution of 5 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 8 Gbp2b/Gbp1 protein in skin of uninfected mice. Slices of skin tissue of females of BALB/c (A) , STS (B) , CcS-5 (C) , CcS-20 (D) , and O20 (E) mice were stained with the rabbit anti-Gbp1 Polyclonal antibody (PA5-23509, Thermo Fisher Scientific, Rockford, IL, USA) diluted 1:100 and anti-rabbit-AlexaFluor-647 (cat. no. 711-605-152; Jackson ImmunoResearch, West Grove, PA) diluted 1:500. Nuclei of the cells were stained with bisBenzimide H33258 (Sigma-Aldrich, St. Louis, MO, USA) 10 mg per 1 ml diluted 1:1,000. Images were captured with microscope Leica DM6000 objective HCX PL Apo 40x/0.75 PH2 and color camera Leica DFC490. Evaluation of images was done with Fiji ImageJ 1.51n. Figures are representatives of data from 8 to 12 mice (see Materials and Methods ) in 3 of them 10 fields (320.66 x 239.57 uM) from each mouse were analyzed, in the rest one field was analyzed to verify the results. Green arrows show Gbp2b/Gbp1 protein (yellow color), cell nuclei are stained in blue.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 9 Gbp2b/Gbp1 protein and Leishmania major parasites in skin of infected mice. Slices of skin tissue of females of BALB/c (A) , STS (B) , CcS-5 (C) , CcS-20 (D) , and O20 (E) mice infected for 8 weeks with L. major were stained with the anti- Leishmania lipophosphoglycan mouse monoclonal antibody (cat. no. CLP003A, Cedarlane, Hornby, Canada) and TRITC labeled IgM (115-025-020, Jackson ImmunoResearch, West Grove, PA) all diluted 1:500 and the rabbit anti-Gbp1 Polyclonal antibody (PA5-23509, Thermo Fisher Scientific, Rockford, IL, USA) diluted 1:100 and anti-rabbit-AlexaFluor-647 (cat. no. 711-605-152; Jackson ImmunoResearch, West Grove, PA) diluted 1:500. Nuclei of the cells were stained with bisBenzimide H33258 (Sigma-Aldrich, St. Louis, MO, USA) 10 mg per 1 ml diluted 1:1,000. Images were captured with microscope Leica DM6000 objective HCX PL Apo 40x/0.75 PH2 and color camera Leica DFC490. Evaluation of images was done with Fiji ImageJ 1.51n. Figures are representatives of data from 8 to 11 mice (see Materials and Methods ) in 3 of them 10 fields (320.66 x 239.57 uM) from each mouse were analyzed, in the rest one field was analyzed to verify the results. White arrow shows L. major amastigotes (red color), green arrows show Gbp2b/Gbp1 protein (yellow color), red arrows point to amastigotes co-localized with Gbp2b/Gbp1, whereas yellow arrows show either Gbp2b/Gbp1 surrounding parasite clusters or stretch of parasites and Gbp2b/Gbp1. Cell nuclei are stain

PA5-23509

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