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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-21034 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KPNA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is rat heart tissue lysate.
- Concentration
- 1 mg/mL
Submitted references Active nuclear import of the deacetylase Sirtuin-2 is controlled by its C-terminus and importins.
Eldridge MJG, Pereira JM, Impens F, Hamon MA
Scientific reports 2020 Feb 10;10(1):2034
Scientific reports 2020 Feb 10;10(1):2034
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat heart tissue lysate using a KPNA2 polyclonal antibody (Product # PA5-21034) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of KPNA2 expression in Daudi cell lysate with KPNA2 Polyclonal Antibody (Product # PA5-21034) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of KPNA2 in rat heart tissue lysate with KPNA2 Polyclonal Antibody (Product # PA5-21034) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of KPNA2 was achieved by transfecting HEK-293 with KPNA2 specific siRNAs (Silencer® select Product # s7921, s7920). Western blot analysis (Fig. a) was performed using whole cell extracts from the KPNA2 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with KPNA2 Polyclonal Antibody (Product # PA5-21034, 1:2500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to KPNA2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-KPNA2 Polyclonal Antibody (Product # PA5-21034) and a 58 kDa band corresponding to KPNA2 was observed across the panel tested except in Mouse and Rat Thymus which are low expressors of KPNA2. Whole cell extracts of HEK-293 (Lane 1), A549 (Lane 2), HeLa (Lane 3), NIH/3T3 (Lane 4), LNCaP (Lane 5) and tissue extracts of (20 µg lysate) Mouse Testis (Lane 6), Mouse Thymus (Lane 7), Rat Testis (Lane 8) and Rat Thymus (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of KPNA2 in human heart tissue with KPNA2 Polyclonal Antibody (Product # PA5-21034) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of KPNA2 in human heart tissue with KPNA2 Polyclonal Antibody (Product # PA5-21034) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 SIRT2 interacts with multiple importins. SIRT2-GFP or GFP alone where immunoprecipitated using GFP-Trap(r) agarose beads for 1 hr. Cell lysates (INPUT) and IP fractions were immunoblotted using antibodies against GFP and (A) KPNA2, IPO7, TNPO1 or, (B) IPO13 and CBP which served as controls. Images are representative of 3 independent experiments. Uncropped blots are presented in Supplementary S3.
