H00000338-M14

antibody from Abnova Corporation

Targeting: APOB

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000338-M14

Provider

Abnova Corporation

Product name

APOB monoclonal antibody (M14), clone 3E5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant APOB.

Antigen sequence

EEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSS
GVPGTADSRSATRINCKVELEVPQLCSFILKTSQC
TLKEVYGFNPEGKALLKKTKNSEEFAAAMS


Isotype

IgG

Antibody clone number

3E5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of APOB expression in transfected 293T cell line by APOB monoclonal antibody (M14), clone 3E5.Lane 1: APOB transfected lysate (Predicted MW: 11.11 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged APOB is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol