Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 11-1347-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD134 (OX40) Monoclonal Antibody (ACT35 (ACT-35)), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The ACT35 monoclonal antibody reacts with human CD134, also known as OX-40. A member of the TNF receptor superfamily, CD134/OX-40 is a 50 kDa type I membrane glycoprotein expressed by activated T lymphocytes. The interaction of CD134 with OX-40L has been implicated in T cell-dependent humoral response, regulation of primary T cell expansion, survival of T cells, size of the memory T cell pool and regulation of tolerance in the CD4^+ T cell compartment. Applications Reported: This ACT35 (ACT-35) antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections. Applications Tested: This ACT35 (ACT-35) antibody has been pre-titrated and tested by flow cytometric analysis of 3-day PHA-activated human normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- ACT35 (ACT-35)
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references IL-7 abrogates suppressive activity of human CD4+CD25+FOXP3+ regulatory T cells and allows expansion of alloreactive and autoreactive T cells.
Heninger AK, Theil A, Wilhelm C, Petzold C, Huebel N, Kretschmer K, Bonifacio E, Monti P
Journal of immunology (Baltimore, Md. : 1950) 2012 Dec 15;189(12):5649-58
Journal of immunology (Baltimore, Md. : 1950) 2012 Dec 15;189(12):5649-58
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of PHA-stimulated (purple histogram) and unstimulated (blue histogram) human PBMCs with Anti-Human CD134 FITC. Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye