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LearnTA328659
antibody from OriGene
Targeting: NECTIN1
CD111, CLPED1, ED4, HIgR, HVEC, OFC7, PRR, PRR1, PVRL1, PVRR1, SK-12
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA328659 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-Nectin-1 (extracellular)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-Nectin-1 (extracellular)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- NECTIN1
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
Submitted references Tuberous sclerosis complex exhibits a new renal cystogenic mechanism.
Bissler JJ, Zadjali F, Bridges D, Astrinidis A, Barone S, Yao Y, Redd JR, Siroky BJ, Wang Y, Finley JT, Rusiniak ME, Baumann H, Zahedi K, Gross KW, Soleimani M
Physiological reports 2019 Jan;7(2):e13983
Physiological reports 2019 Jan;7(2):e13983
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: 1-2. Anti-Nectin-1 (extracellular) antibody, (1:200). 3-4. Anti-Nectin-1 (extracellular) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat T-cell leukemia (lanes 1 and 3) and human MEG-01 chronic myelogenous leukemia (lanes 2 and 4) cell lysates: 1-2. Anti-Nectin-1 (extracellular) antibody, (1:200). 3-4. Anti-Nectin-1 (extracellular) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of Nectin-1 in rat PC12 cells. Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-Nectin-1 (extracellular) antibody, (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of the two images.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of Nectin-1 in rat cerebellum. Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nectin-1 (extracellular) antibody, (1:100). A. Nectin-1 staining (red) is apparent in Purkinje neurons and their dendritic tree (arrow). B. Cell nuclei in the same section are visualized with DAPI (blue). C. Merge of the two images.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Indirect flow cytometry analysis of live intact human THP-1 monocytic leukemia cell line: black line, Cells + goat anti-rabbit-AlexaFluor-488 secondary antibody. green line, Cells + Anti-Nectin-1 (extracellular) antibody, (1:15) + goat anti-rabbit-AlexaFluor-488 secondary antibody.
- Validation comment
- FC
