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LearnMAB5758
antibody from Novus Biologicals
Targeting: ING1
p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- MAB5758 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ING1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human and mouse ING1 in Western blot.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse ING1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HT-2 mouse T cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ING1 Monoclonal Antibody (Catalog # MAB5758) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ING1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
