39-9500

antibody from Invitrogen Antibodies

Targeting: GSK3B

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

39-9500

Provider

Invitrogen Antibodies

Product name

GSK3B Monoclonal Antibody (ZG004)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

ZG004

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C

References

Submitted references

MYBL2 in synergy with CDC20 promotes the proliferation and inhibits apoptosis of gastric cancer cells.

Deng Q, Wu L, Li Y, Zou L
Advances in clinical and experimental medicine : official organ Wroclaw Medical University 2021 Sep;30(9):957-966

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Interruption of beta-catenin signaling reduces neurogenesis in Alzheimer's disease.

He P, Shen Y
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 May 20;29(20):6545-57

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NGF-stimulated PC12 cell lysates using Zymed (R) Ms anti-GSK3beta (Product # 39-9500).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NGF-stimulated PC12 cell lysates using Zymed (R) Ms anti-GSK3beta (Product # 39-9500).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (20 µg lysate) of HEK-293 (lane 1), K562 (lane 2), A-431 (lane 3), PC-3 (lane 4), U-87 MG (lane 5) and Jurkat (lane 6). The blots were probed with Anti-GSK3 Beta Mouse Monoclonal Antibody (Product # 39-9500, 1:250 dilution) and detected by chemiluminescence Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 48 kDa band corresponding to GSK3 Beta was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GSK3 Beta showing staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a GSK3 Beta Mouse Monoclonal Antibody (clone ZG004) (Product # 39-9500) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL