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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [2]
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- Product number
- MAB8110 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CXCR2/IL-8RB Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat CXCR2/IL-8 RB in direct ELISAs. In Flow Cytometry, stains HEK293 cells transfected with rat CXCR2/IL-8 RB, but does not stain non-transfected cells.
- Reactivity
- Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of CXCR2/IL-8 RB in Rat Blood Granulocytes by Flow Cytometry. Rat peripheral blood granulocytes were stained with Mouse Anti-Rat CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB8110, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of CXCR2/IL-8 RB in HEK293 Human Cell Line Transfected with Rat CXCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) rat CXCR2/IL-8 RB or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB8110) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB004). View our protocol for Staining Membrane-associated Proteins.
