Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- 12-1829-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD182 (CXCR2) Monoclonal Antibody (eBio5E8-C7-F10 (5E8-C7-F10)), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBio5E8-C7-F10 monoclonal antibody reacts with human CD182 (CXCR2, IL-8R beta). CD182 is a 67-70 kDa member of the seven-transmembrane spanning G-protein coupled receptor (GPCR) family. CD182 is expressed as a homodimer, or a heterodimer with CD181 (CXCR1, IL-8R alpha) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it's ligands, which include IL-8, NAP-2, GCP-2 and GRO alpha, -beta and -gamma, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.
- Conjugate
- Yellow dye
- Antibody clone number
- eBio5E8-C7-F10 (5E8-C7-F10)
- Concentration
- 5 µL/Test
Submitted references Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.
IL-33/ST2 pathway regulates neutrophil migration and predicts outcome in patients with severe alcoholic hepatitis.
Ten weeks of high-intensity interval walk training is associated with reduced disease activity and improved innate immune function in older adults with rheumatoid arthritis: a pilot study.
n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.
Habitual physical activity is associated with the maintenance of neutrophil migratory dynamics in healthy older adults.
The CXC chemokine receptor 2, CXCR2, is the putative receptor for ELR+ CXC chemokine-induced angiogenic activity.
The biologic role of interleukin-8: functional analysis and expression of CXCR1 and CXCR2 on human eosinophils.
Chemokine antagonists that discriminate between interleukin-8 receptors. Selective blockers of CXCR2.
Monoclonal antibodies detect different distribution patterns of IL-8 receptor A and IL-8 receptor B on human peripheral blood leukocytes.
Structure, genomic organization, and expression of the human interleukin-8 receptor B gene.
Genkel V, Dolgushin I, Baturina I, Savochkina A, Kuznetsova A, Pykhova L, Shaposhnik I
International journal of inflammation 2021;2021:6695468
International journal of inflammation 2021;2021:6695468
IL-33/ST2 pathway regulates neutrophil migration and predicts outcome in patients with severe alcoholic hepatitis.
Artru F, Bou Saleh M, Maggiotto F, Lassailly G, Ningarhari M, Demaret J, Ntandja-Wandji LC, Pais de Barros JP, Labreuche J, Drumez E, Helou DG, Dharancy S, Gantier E, Périanin A, Chollet-Martin S, Bataller R, Mathurin P, Dubuquoy L, Louvet A
Journal of hepatology 2020 Jun;72(6):1052-1061
Journal of hepatology 2020 Jun;72(6):1052-1061
Ten weeks of high-intensity interval walk training is associated with reduced disease activity and improved innate immune function in older adults with rheumatoid arthritis: a pilot study.
Bartlett DB, Willis LH, Slentz CA, Hoselton A, Kelly L, Huebner JL, Kraus VB, Moss J, Muehlbauer MJ, Spielmann G, Kraus WE, Lord JM, Huffman KM
Arthritis research & therapy 2018 Jun 14;20(1):127
Arthritis research & therapy 2018 Jun 14;20(1):127
n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.
Wu B, Wang L, Jiang L, Dong L, Xu F, Lu Y, Jin J, Wang Z, Liang G, Shan X
Molecular medicine reports 2018 Jan;17(1):179-185
Molecular medicine reports 2018 Jan;17(1):179-185
Habitual physical activity is associated with the maintenance of neutrophil migratory dynamics in healthy older adults.
Bartlett DB, Fox O, McNulty CL, Greenwood HL, Murphy L, Sapey E, Goodman M, Crabtree N, Thøgersen-Ntoumani C, Fisher JP, Wagenmakers AJ, Lord JM
Brain, behavior, and immunity 2016 Aug;56:12-20
Brain, behavior, and immunity 2016 Aug;56:12-20
The CXC chemokine receptor 2, CXCR2, is the putative receptor for ELR+ CXC chemokine-induced angiogenic activity.
Addison CL, Daniel TO, Burdick MD, Liu H, Ehlert JE, Xue YY, Buechi L, Walz A, Richmond A, Strieter RM
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 1;165(9):5269-77
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 1;165(9):5269-77
The biologic role of interleukin-8: functional analysis and expression of CXCR1 and CXCR2 on human eosinophils.
Petering H, Götze O, Kimmig D, Smolarski R, Kapp A, Elsner J
Blood 1999 Jan 15;93(2):694-702
Blood 1999 Jan 15;93(2):694-702
Chemokine antagonists that discriminate between interleukin-8 receptors. Selective blockers of CXCR2.
Jones SA, Dewald B, Clark-Lewis I, Baggiolini M
The Journal of biological chemistry 1997 Jun 27;272(26):16166-9
The Journal of biological chemistry 1997 Jun 27;272(26):16166-9
Monoclonal antibodies detect different distribution patterns of IL-8 receptor A and IL-8 receptor B on human peripheral blood leukocytes.
Chuntharapai A, Lee J, Hébert CA, Kim KJ
Journal of immunology (Baltimore, Md. : 1950) 1994 Dec 15;153(12):5682-8
Journal of immunology (Baltimore, Md. : 1950) 1994 Dec 15;153(12):5682-8
Structure, genomic organization, and expression of the human interleukin-8 receptor B gene.
Sprenger H, Lloyd AR, Lautens LL, Bonner TI, Kelvin DJ
The Journal of biological chemistry 1994 Apr 15;269(15):11065-72
The Journal of biological chemistry 1994 Apr 15;269(15):11065-72
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0048-42) (left) or Anti-Human CD56 (NCAM) APC (right) and Anti-Human CD182 (CXCR2) PE. Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye