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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
- Other assay [2]
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- Product number
- 14-1829-85 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD182 (CXCR2) Monoclonal Antibody (eBio5E8-C7-F10 (5E8-C7-F10)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBio5E8-C7-F10 monoclonal antibody reacts with human CD182 (CXCR2, IL-8Rbeta). CD182 is a 67-70 kDa member of the seven-transmembrane spanning G-protein coupled receptor (GPCR) family. CD182 is expressed as a homodimer, or a heterodimer with CD181 (CXCR1, IL-8Ralpha) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it's ligands, which include IL-8, NAP-2, GCP-2 and GRO-alpha, -beta and -gamma, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.
- Antibody clone number
- eBio5E8-C7-F10 (5E8-C7-F10)
- Concentration
- 0.5 mg/mL
Submitted references n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.
Type I IFNs induce anti-tumor polarization of tumor associated neutrophils in mice and human.
Anomalous type 17 response to viral infection by CD8+ T cells lacking T-bet and eomesodermin.
Wu B, Wang L, Jiang L, Dong L, Xu F, Lu Y, Jin J, Wang Z, Liang G, Shan X
Molecular medicine reports 2018 Jan;17(1):179-185
Molecular medicine reports 2018 Jan;17(1):179-185
Type I IFNs induce anti-tumor polarization of tumor associated neutrophils in mice and human.
Andzinski L, Kasnitz N, Stahnke S, Wu CF, Gereke M, von Köckritz-Blickwede M, Schilling B, Brandau S, Weiss S, Jablonska J
International journal of cancer 2016 Apr 15;138(8):1982-93
International journal of cancer 2016 Apr 15;138(8):1982-93
Anomalous type 17 response to viral infection by CD8+ T cells lacking T-bet and eomesodermin.
Intlekofer AM, Banerjee A, Takemoto N, Gordon SM, Dejong CS, Shin H, Hunter CA, Wherry EJ, Lindsten T, Reiner SL
Science (New York, N.Y.) 2008 Jul 18;321(5887):408-11
Science (New York, N.Y.) 2008 Jul 18;321(5887):408-11
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (left) or 0.25 µg of Anti-Human CD182 (CXCR2) Purified (right) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Cells in the large scatter population were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
