656608

antibody from BioLegend

Targeting: INPP5D hp51CN, SHIP, SHIP1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

656608

Provider

BioLegend

Proper citation

BioLegend Cat#656608, RRID:AB_2563145

Product name

Alexa Fluor® 647 anti-SHIP-1

Antibody type

Monoclonal

Antigen

Human SHIP fusion protein in CFA/IFA

Description

SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes key signaling lipid PI(3,4,5)P(3) to PI(3,4)P(2). SHIP-1 is located in the cytoplasm and is recruited to the cell surface upon receptor ligation. It plays critical roles in hematopoietic cell proliferation, activation, differentiation, and survival by regulating PI3K and Akt pathways. Aberrant regulation of PI3K signaling can induce autoimmune diseases and cancer formation.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Red dye

Antibody clone number

P1C1-A5

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

C57BL/6 mouse bone marrow cells were stimulated with or without LPS for 48 hours, then fixed and permeabilized with Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002). Cells were then stained with SHIP-1 (clone P1C1-A5) Alexa Fluor® 647 (filled histogram for LPS stimulated, open histogram with purple solid line for unstimulated) or mouse IgG1, κ Alexa Fluor® 647 isotype control (dashed line for unstimulated, dotted line for stimulated). Data shown was gated on myeloid cell population.

Conjugate

Red dye