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LearnPA1-044
antibody from Invitrogen Antibodies
Targeting: DLG1
dJ1061C18.1.1, DLGH1, hdlg, SAP-97, SAP97
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [3]
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- Product number
- PA1-044 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SAP97 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-044 detects synapse-associated protein 97 (SAP97) from rat and human tissues. This antibody does not detect other synapse-associated protein family members.
- Concentration
- 1 mg/mL
Submitted references Schizophrenia-associated SAP97 mutations increase glutamatergic synapse strength in the dentate gyrus and impair contextual episodic memory in rats.
SAP97 blocks the RXR ER retention signal of NMDA receptor subunit GluN1-3 through its SH3 domain.
Characteristics of the turnover of uncoupling protein 3 by the ubiquitin proteasome system in isolated mitochondria.
Effect of rasagiline on the molecular composition of the excitatory postsynaptic density.
Distinct levels of dopamine denervation differentially alter striatal synaptic plasticity and NMDA receptor subunit composition.
PSD93 regulates synaptic stability at neuronal cholinergic synapses.
Kay Y, Tsan L, Davis EA, Tian C, Décarie-Spain L, Sadybekov A, Pushkin AN, Katritch V, Kanoski SE, Herring BE
Nature communications 2022 Feb 10;13(1):798
Nature communications 2022 Feb 10;13(1):798
SAP97 blocks the RXR ER retention signal of NMDA receptor subunit GluN1-3 through its SH3 domain.
Hong X, Avetisyan M, Ronilo M, Standley S
Biochimica et biophysica acta 2015 Feb;1853(2):489-99
Biochimica et biophysica acta 2015 Feb;1853(2):489-99
Characteristics of the turnover of uncoupling protein 3 by the ubiquitin proteasome system in isolated mitochondria.
Mookerjee SA, Brand MD
Biochimica et biophysica acta 2011 Nov;1807(11):1474-81
Biochimica et biophysica acta 2011 Nov;1807(11):1474-81
Effect of rasagiline on the molecular composition of the excitatory postsynaptic density.
Gardoni F, Zianni E, Eramo A, Canonico PL, Di Luca M
European journal of pharmacology 2011 Nov 30;670(2-3):458-63
European journal of pharmacology 2011 Nov 30;670(2-3):458-63
Distinct levels of dopamine denervation differentially alter striatal synaptic plasticity and NMDA receptor subunit composition.
Paillé V, Picconi B, Bagetta V, Ghiglieri V, Sgobio C, Di Filippo M, Viscomi MT, Giampà C, Fusco FR, Gardoni F, Bernardi G, Greengard P, Di Luca M, Calabresi P
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Oct 20;30(42):14182-93
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Oct 20;30(42):14182-93
PSD93 regulates synaptic stability at neuronal cholinergic synapses.
Parker MJ, Zhao S, Bredt DS, Sanes JR, Feng G
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Jan 14;24(2):378-88
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Jan 14;24(2):378-88
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of SAP97 on extract from COS cells transfected with rat SAP97 using Product # PA1-044.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane extracts (30 µglysate) of HeLa (Lane 1), and MCF-7 (Lane 2). The blots were probed with Rabbit Anti-SAP97 Polyclonal Antibody (Product # PA1-044, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 110 kDa band corresponding to SAP97 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane extracts (30 µglysate) of HeLa (Lane 1), and MCF-7 (Lane 2). The blots were probed with Rabbit Anti-SAP97 Polyclonal Antibody (Product # PA1-044, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 110 kDa band corresponding to SAP97 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
