MAB64891

antibody from Novus Biologicals

Targeting: ACAN AGC1, CSPG1, CSPGCP, MSK16

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB64891

Provider

Novus Biologicals

Product name

Mouse Monoclonal Aggrecan Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Aggrecan in direct ELISAs and Western blots. It specifically recognizes the C-terminal fragment of recombinant human Aggrecan released following cleavage by ADAMTS5.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Aggrecan (ADAMTS5-cleaved) by Western Blot. Western blot shows recombinant human Aggrecan N-terminal and C-terminal fragments. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Aggrecan Monoclonal Antibody (Catalog # MAB64891) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for the Aggrecan C-terminal fragment at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.