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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09411PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09411PU-S, RRID:AB_2035874
- Product name
- anti RBL2 / p130 (SPA310)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to the Spa310 sequence of pRb2/p130 protein. A residue of cysteine was added to facilitate coupling
- Reactivity
- Human, Rat, Bovine, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µl
- Concentration
- 1.39 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using affinity purified anti-Spa310 antibody shows detection of endogenous pRb2/p130 protein in whole LNCaP cell extracts. The band at ~130 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of pRb2/p130. The membrane was blocked overnight with a milk buffer at 4° C. Primary antibody was diluted 1:500 and reacted with the membrane overnight at 4° C. ECL was used for detection.
