PA5-19116

antibody from Invitrogen Antibodies

Targeting: CAV3 LGMD1C, LQT9, VIP-21, VIP21

Antibody data

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Validation data

Product number: PA5-19116 - Provider product page
Provider: Invitrogen Antibodies
Product name: Caveolin 3 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody is predicted to react with bovine, canine and porcine based on sequence homology.
Reactivity: Human, Mouse, Rat, Porcine
Host: Goat
Isotype: IgG
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

CAV3 protein structure - PA5-19116 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Caveolin 3 by a Caveolin 3 monoclonal antibody (Product # PA5-19116) at a concentration of 0.1 µg/mL. Pig Skeletal Muscle (A) and Heart (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot staining of Human Heart lysate using Product # PA5-19116 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Caveolin 3 by a Caveolin 3 monoclonal antibody (Product # PA5-19116) at a concentration of 0.3 µg/mL. Mouse (A) and Rat (B) Heart lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Rat Skeletal Muscle (Lane 3), Mouse Kidney (Lane 4) and Mouse Skin (Lane 5). The blot was probed with anti-Caveolin 3 Polyclonal Antibody (Product # PA5-19116, 0.2µg/mL) and detected by chemiluminescence using Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A27014, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to Caveolin 3 was observed in Mouse Heart, Rat Heart, Rat Skeletal Muscle when compared to Mouse Kidney and Mouse Skin which are reported to be negative for Caveolin 3 expression.