MA1-34594

antibody from Invitrogen Antibodies

Targeting: NEK2 NEK2A, NLK1, PPP1R111, RP67

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-34594

Provider

Invitrogen Antibodies

Product name

NEK2 Monoclonal Antibody (1B6)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: Jurkat cell lysate.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1B6

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NEK2 in Jurkat whole cell extract using a NEK2 monoclonal antibody (Product # MA1-34594).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NEK2 Monoclonal Antibody (1B6) (Product # MA1-34594). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 Monoclonal Antibody (1B6) (Product # MA1-34594) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NEK2 in Jurkat whole cell extract using a NEK2 monoclonal antibody (Product # MA1-34594).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-NEK2 Monoclonal Antibody (Product # MA1-34594) and a 52 kDa band corresponding to NEK2 was observed across all the cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HEK-293 (Lane 1), MCF7 (Lane 2) and Hep G2 (Lane 3) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1.25 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).