IC350A

antibody from Novus Biologicals

Targeting: CXCL12 PBSF, SCYB12, SDF-1a, SDF-1b, SDF1, SDF1A, SDF1B, TLSF-a, TLSF-b, TPAR1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC350A

Provider

Novus Biologicals

Product name

Mouse Monoclonal CXCL12/SDF-1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human and mouse CXCL12/SDF-1 alpha and CXCL12/SDF-1 beta in ELISAs.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

References

Submitted references

Multiple myeloma exploits Jagged1 and Jagged2 to promote intrinsic and bone marrow-dependent drug resistance.

Colombo M, Garavelli S, Mazzola M, Platonova N, Giannandrea D, Colella R, Apicella L, Lancellotti M, Lesma E, Ancona S, Palano MT, Barbieri M, Taiana E, Lazzari E, Basile A, Turrini M, Pistocchi A, Neri A, Chiaramonte R
Haematologica 2020 Jul;105(7):1925-1936

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Follicular helper T cells recruit eosinophils into host liver by producing CXCL12 during Schistosoma japonicum infection.

Chen X, Xu Z, Wei C, Yang X, Xu L, Zhou S, Zhu J, Su C
Journal of cellular and molecular medicine 2020 Feb;24(4):2566-2572

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Distinct Bone Marrow Sources of Pleiotrophin Control Hematopoietic Stem Cell Maintenance and Regeneration.

Himburg HA, Termini CM, Schlussel L, Kan J, Li M, Zhao L, Fang T, Sasine JP, Chang VY, Chute JP
Cell stem cell 2018 Sep 6;23(3):370-381.e5

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"Inflamm-aging" influences immune cell survival factors in human bone marrow.

Pangrazzi L, Meryk A, Naismith E, Koziel R, Lair J, Krismer M, Trieb K, Grubeck-Loebenstein B
European journal of immunology 2017 Mar;47(3):481-492

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Differential cytokine contributions of perivascular haematopoietic stem cell niches.

Asada N, Kunisaki Y, Pierce H, Wang Z, Fernandez NF, Birbrair A, Ma'ayan A, Frenette PS
Nature cell biology 2017 Mar;19(3):214-223

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Downregulation of CXCL12 in mesenchymal stromal cells by TGFβ promotes breast cancer metastasis.

Yu PF, Huang Y, Xu CL, Lin LY, Han YY, Sun WH, Hu GH, Rabson AB, Wang Y, Shi YF
Oncogene 2017 Feb 9;36(6):840-849

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DOCK2 is critical for CD8(+) TCR(-) graft facilitating cells to enhance engraftment of hematopoietic stem and progenitor cells.

Wen Y, Elliott MJ, Huang Y, Miller TO, Corbin DR, Hussain LR, Ratajczak MZ, Fukui Y, Ildstad ST
Stem cells (Dayton, Ohio) 2014 Oct;32(10):2732-43

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The Spiegelmer NOX-A12, a novel CXCL12 inhibitor, interferes with chronic lymphocytic leukemia cell motility and causes chemosensitization.

Hoellenriegel J, Zboralski D, Maasch C, Rosin NY, Wierda WG, Keating MJ, Kruschinski A, Burger JA
Blood 2014 Feb 13;123(7):1032-9

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of CXCL12/SDF-1 in MG-63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human/Mouse CXCL12/SDF-1 APC-conjugated Mono-clonal Antibody (Catalog # IC350A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.