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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 656004 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#656004, RRID:AB_2563007
- Product name
- PE anti-IRF7
- Antibody type
- Monoclonal
- Antigen
- Partial IRF7 recombinant protein (127-503 a.a.)
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- 12G9A36
- Vial size
- 100 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Overnight starved Jurkat cells were treated with or without IFN-α for 6 hours, then fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601). Cells were then stained with IRF7 (clone 12G9A36) PE (solid line with open histogram for untreated cells, filled histogram for stimulated cells) or mouse IgG2b, κ PE isotype control (dash line for unstimulated, dot line for stimulated).
- Conjugate
- Yellow dye
