Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [3]
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-34364-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal NCAM-1/CD56 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (SPM489) [NBP2-34364] - Analysis using the Azide and BSA Free version of NBP2-34364. Staining of human neuro-endocrine tumor stained with CD56 MAb (SPM489).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (SPM489) [NBP2-34364] - Analysis using the Azide and BSA Free version of NBP2-34364. Staining of human colon ganglion stained with CD56 MAb (SPM489).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (SPM489) [NBP2-34364] - Formalin-fixed, paraffin-embedded human colon stained with CD56 Monoclonal Antibody (SPM489)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NCAM-1/CD56 Antibody (SPM489) [NBP2-34364] - Using the PerCP direct conjugate A cell surface stain was performed on SH-SY5Y cells with NCAM-1/CD56 antibody (SPM489) NBP2-34412PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NCAM-1/CD56 Antibody (SPM489) [NBP2-34364] - A surface stain was performed on U-87 cells with NCAM-1/CD56 [SPM489] Antibody NBP2-34412AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.