- 56-0566-42 - Invitrogen Antibodies NCAM1 antibody

Submitted references Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus.

Satria RD, Huang TW, Jhan MK, Shen TJ, Tseng PC, Wang YT, Yang ZY, Hsing CH, Lin CF
Journal of immunology research 2021;2021:6654617

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Kim YD, Park SM, Ha HC, Lee AR, Won H, Cha H, Cho S, Cho JM
Journal of Cancer 2020;11(14):4059-4072

Myogenic Progenitor Cell Lineage Specification by CRISPR/Cas9-Based Transcriptional Activators.

Kwon JB, Vankara A, Ettyreddy AR, Bohning JD, Gersbach CA
Stem cell reports 2020 May 12;14(5):755-769

The Master Regulator Protein BAZ2B Can Reprogram Human Hematopoietic Lineage-Committed Progenitors into a Multipotent State.

Arumugam K, Shin W, Schiavone V, Vlahos L, Tu X, Carnevali D, Kesner J, Paull EO, Romo N, Subramaniam P, Worley J, Tan X, Califano A, Cosma MP
Cell reports 2020 Dec 8;33(10):108474

Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.

Yang C, Siebert JR, Burns R, Gerbec ZJ, Bonacci B, Rymaszewski A, Rau M, Riese MJ, Rao S, Carlson KS, Routes JM, Verbsky JW, Thakar MS, Malarkannan S
Nature communications 2019 Sep 2;10(1):3931

Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56.

Zou F, Lu L, Liu J, Xia B, Zhang W, Hu Q, Liu W, Zhang Y, Lin Y, Jing S, Huang M, Huang B, Liu B, Zhang H
Nature communications 2019 Sep 11;10(1):4109

Memory B Cells Activate Brain-Homing, Autoreactive CD4(+) T Cells in Multiple Sclerosis.

Jelcic I, Al Nimer F, Wang J, Lentsch V, Planas R, Jelcic I, Madjovski A, Ruhrmann S, Faigle W, Frauenknecht K, Pinilla C, Santos R, Hammer C, Ortiz Y, Opitz L, Grönlund H, Rogler G, Boyman O, Reynolds R, Lutterotti A, Khademi M, Olsson T, Piehl F, Sospedra M, Martin R
Cell 2018 Sep 20;175(1):85-100.e23

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Normal human peripheral blood cells cells were stained with CD3 Monoclonal Antibody, FITC (Product # 11-0037-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 700 (Product # 56-4714-80) (left) or CD56 Monoclonal Antibody, Alexa Fluor 700 (right). Cells in the lymphocyte gate were used for analysis.
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Characterization of Myogenic Progenitors Derived from iPSCs via VP64 dCas9 VP64 -Mediated Activation of Endogenous PAX7 or Exogenous PAX7 cDNA Expression (A) Relative amounts of total PAX7 mRNA was determined by qRT-PCR using primers complementary to sequences present in the gene body. (B) Endogenous PAX7 mRNA was detected using primers complementary to sequences in the 3' UTR of either isoform PAX7-A or PAX7-B . (C) The mRNA expression levels of myogenic markers MYF5 , MYOD , and MYOG during the expansion phase. (D) Immunofluorescence staining of early and mature myogenic markers MYF5, MYOD, and MYOG, and myosin heavy chain (MHC). (E) Representative FACS analysis of CD29 and CD56 surface marker expression during the expansion phase. (F) Mean fluorescence intensity (MFI) of CD56 staining intensity across treatments. All p values were determined by one-way ANOVA followed by Tukey's post hoc test (mean +- SEM, n = 3 independent replicates).
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4 Immune profiling in DENV-treated whole blood cells 24 h postincubation. Following DENV (MOI = 1) coculture in 100 mu l of WB ex vivo for 24 h, (a) representative flow cytometric analysis and gating of various cells obtained from five cases, performed by staining for specific cell surface markers (CD4, CD8, CD11c, CD14, CD16, CD19, CD25, CD56, CD62L, and HLA-DR), in the DENV-infected and mock groups showed (b) the changes in the expression of specific immune cell populations as noted. (c) The results are shown as a percentage of the mean +- SD obtained from five cases. * p < 0.05, ** p < 0.01, and *** p < 0.001, compared to the mock group. R: region; WBC: white blood cell; bri: bright.
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 CD56 enhanced anti-apoptosis capability of CAR-T cells. a Left panel, the representative flow plots of apoptotic cell proportion in Her2-CAR-T, PTL-Her2-CAR-T, and CD56-Her2-CAR-T cells after culture for 6 days with cytokine deprivation. Right panel, the percentage of Annexin V - and PI - cells gated as in left panel. b Left panel, the representative flow plots of apoptotic cell proportion induced by TNF-alpha and FasL (10 ng ml -1 ) in various CAR-T cells. Right panel, the percentage of Annexin V - and PI - cells gated as in the left panel. ** P < 0.01, **** P < 0.0001 (two-tailed Student's t -test). All presented data summarizes mean +- SEM. Data shown are representative data from three independent experiments. The source data underlying a , b are provided as a Source Data file
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 Transcriptional signature of functionally mature NK cells. a Representative genes associated with functionally mature NK cells were grouped in four different categories and plotted via violin plots of the BM sample. The y -axis represents log-normalized expression value. b The expression of ANXA1 , ANXA2 , and ANXA6 in each cluster of the blood sample was shown as violin plots. The y -axis represents log-normalized expression value. c The intracellular protein level of Annexin A1 in different NK cell subsets from blood at steady state was assessed by flow cytometry. The representative histogram was shown on the left. FMO stands for fluorescence minus one. The mean fluorescence intensity (MFI) of Annexin A1 in each NK subsets was normalized to CD56 bright NK subset and shown on the right. n = 5 from three independent experiments. Error bars were shown as standard deviation. One-way ANOVA was used for the statistical analysis. * P < 0.05; ** p < 0.01; *** p < 0.001. d Isolated NK cells from blood were stimulated with medium, IL-12, and IL-18 (10/10 ng/mL), or PMA and ionomycin (50/500 ng/mL) for 6 h. The protein level of Annexin A1 was assessed following stimulation. The MFI of Annexin A1 in each condition was normalized to the medium-only NK cells. n = 5 from three independent experiments. Error bars were shown as standard deviation. One-way ANOVA was used for the statistical analysis. * P < 0.05; ** p < 0.01; *** p < 0.001. e Isolated NK cells from blood were stimulate
Conjugate: Near infrared dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 CG-745 increases helper T cells, cytotoxic T cells and natural killer T cells, and decreases Treg: (A) hPBMCs were incubated with CG (CG-745) for 36 hours and a subset of hPBMCs was analyzed using the antibodies indicated in the text; (B) hPBMCs were co-cultured with Huh7, Hep3B, HepG2 or PLC/PRF/5 cells for 36 hours with or without CG, and a subset of hPBMCs was analyzed by Attune Nxt (Invitrogen, USA).
Conjugate: Near infrared dye

56-0566-42

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