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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- MA5-15107 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD56 Monoclonal Antibody (G.611.8)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- G.611.8
- Vial size
- 200 µL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CD56 Monoclonal Antibody (G.611.8)(Product # MA5-15107) and a 150kDa band corresponding to CD56 was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), IMR-32 (Lane 2), NK-92 (Lane 3), MCF7 (Lane 4), Hep G2 (Lane 5), Neuro-2a (Lane 6), PC-12 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). A streak like pattern was observed in the positive cell lines
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD56 (NCAM) in extracts from human cerebellum using CD56 (NCAM) monoclonal antibody (Product # MA5-15107).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CD56 Monoclonal Antibody (G.611.8)(Product # MA5-15107) and a 150kDa band corresponding to CD56 was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of SH-SY5Y (Lane 1), IMR-32 (Lane 2), NK-92 (Lane 3), MCF7 (Lane 4), Hep G2 (Lane 5), Neuro-2a (Lane 6), PC-12 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). A streak like pattern was observed in the positive cell lines
