Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [2]
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Validation data
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- Product number
- STJ73233 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-PRKD1 antibody (383-395) (STJ73233)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-PRKD1 (383-395) is suitable for use in ELISA, Western Blot and Immunocytochemistry research applications.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
QDPDPDHEDANRT
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20 on receipt and minimise freeze-thaw cycles.
- Handling
- NA
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Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1, Human DYKDDDDK-tagged PKD2 or Human GST-tagged PKD3 probed with STJ73233 (0. 1ug/ml) in top panel, probed with NA NA NA anti-DYKDDDDK in middle panel and probed with anti-GST in bottom panel. Data kindly obtained from Dr Peter Storz, Mayo Clinic, USA.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- HEK293 lysate overexpressing Human DYKDDDDK-tagged PKD1 was used to immunoprecipitate PKD1 with 2ug STJ73233. The precipitate was subsequently probed in Western blot using STJ73233 at 1ug/ml. The secondary anti-goat picks up the heavy chain of STJ73233 used for the immunoprecipitation (annotated as IgG). Data kindly obtained from Dr Peter Storz, Mayo Clinic, USA
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA