orb379501

antibody from Biorbyt

Targeting: ERG erg-3, p55

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

orb379501

Provider

Biorbyt

Product name

Anti-ERG antibody

Antibody type

Monoclonal

Description

Rabbit monoclonal antibody to ERG

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 μl

Storage

Store at 4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Western blot

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Western blot analysis of Jurkat cell lysates using ERG antibody (primary antibody dilution at 1:1000)

Other comments

Jurkat, CRC, PC-3M, human colon cancer tissue, mouse heart tissue, mouse brain tissue

Immunocytochemistry

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Immunofluorescence analysis of ERG in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS using ERG antibody

Other comments

Jurkat, CRC, PC-3M, human colon cancer tissue, mouse heart tissue, mouse brain tissue

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

IF analysis of ERG in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS using ERG antibody

Other comments

Jurkat, CRC, PC-3M, human colon cancer tissue, mouse heart tissue, mouse brain tissue

Immunohistochemistry

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse heart tissue tissue using ERG antibody

Other comments

Jurkat, CRC, PC-3M, human colon cancer tissue, mouse heart tissue, mouse brain tissue

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human colon cancer tissue using ERG antibody

Other comments

Jurkat, CRC, PC-3M, human colon cancer tissue, mouse heart tissue, mouse brain tissue

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

Immunohistochemical staining of mouse brain tissue using ERG antibody

Other comments

Jurkat, CRC, PC-3M, human colon cancer tissue, mouse heart tissue, mouse brain tissue