TA504359

antibody from OriGene

Targeting: PPP1R15A GADD34

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504359

Provider

OriGene

Proper citation

OriGene Cat#TA504359, RRID:AB_11139902

Product name

PPP1R15A (GADD34) mouse monoclonal antibody, clone OTI1E1 (formerly 1E1)

Antibody type

Monoclonal

Description

PPP1R15A (GADD34) mouse monoclonal antibody, clone OTI1E1 (formerly 1E1)

Host

Mouse

Conjugate

Unconjugated

Epitope

PPP1R15A

Isotype

IgG

Antibody clone number

OTI1E1

Vial size

100 µl

Concentration

0.89 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R15A (RC200581, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R15A.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-PPP1R15A monoclonal antibody.(1:200)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504359)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504359)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PPP1R15A antibody(TA504359), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-PPP1R15A antibody(TA504359),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC