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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB2515 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal MKK3/MEK3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human MKK3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human MKK6 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC, as supplied. 1 month, 2 to 8 degreesC under sterile conditions after opening. 6 months, -20 to -70 degreesC under sterile conditions after opening.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human/Mouse/Rat MKK3 by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line, TS1 mouse helper T cell line, and NRK rat normal kidney cell. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat MKK3 Monoclonal Antibody (Catalog # MAB2515) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MKK3 and MKK6 (2 ng/lane) were included. A specific band for MKK3 was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
