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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- NBP2-43701 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Enolase 1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Enolase 1 Antibody (186) [NBP2-43701] - Analysis of A. 30 ug A549 whole cell lysate/extract B. 30 ug H1299 whole cell lysate/extract C. 30 ug HCT116 whole cell lysate/extract 10 % SDS-PAGE ENO1 antibody [186] dilution: 1:10000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Enolase 1 Antibody (186) [NBP2-43701] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [186].
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Enolase 1 Antibody (186) [NBP2-43701] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 Antibody (186) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
