50-0919-42

antibody from Invitrogen Antibodies

Targeting: LRP1 A2MR, APOER, APR, CD91, IGFBP-3R, IGFBP3R1, LRP, LRP1A

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

50-0919-42

Provider

Invitrogen Antibodies

Product name

CD91 Monoclonal Antibody (A2MR-a2), eFluor™ 660, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The A2MR-a2 monoclonal antibody reacts with human CD91, also known as LRP-1 and alpha-2 macroglobulin receptor. CD91 is a 600 kDa, type 1 transmembrane protein consisting of an alpha and beta chain. The alpha chain interacts with multiple structurally and functionally diverse ligands and the beta chain contains 2 tyrosine phosphorylation motifs capable of interacting with adaptor and signaling proteins. Binding of ligands mediates ligand endocytosis and trafficking to lysosomes. This receptor is expressed by cells in the monocytic lineage, dendritic cells, hepatocytes, firbroblasts, adipocytes, neurons, and syncytiotrophoblast. CD91 is involved in cross-presenting antigens via MHC class I molecules on antigen presenting cells after endocytosis of heat shock protein-peptide complexes. It also plays a role in cell migration and is involved in activating signaling cascades.

Antibody clone number

A2MR-a2

Concentration

5 µL/Test

References

Submitted references

Accelerated Wound Healing by Fibroblasts Differentiated from Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells in a Pressure Ulcer Animal Model.

Yoon D, Yoon D, Sim H, Hwang I, Lee JS, Chun W
Stem cells international 2018;2018:4789568

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An AXL/LRP-1/RANBP9 complex mediates DC efferocytosis and antigen cross-presentation in vivo.

Subramanian M, Hayes CD, Thome JJ, Thorp E, Matsushima GK, Herz J, Farber DL, Liu K, Lakshmana M, Tabas I
The Journal of clinical investigation 2014 Mar;124(3):1296-308

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LRP-1: a checkpoint for the extracellular matrix proteolysis.

Etique N, Verzeaux L, Dedieu S, Emonard H
BioMed research international 2013;2013:152163

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CD91-dependent programming of T-helper cell responses following heat shock protein immunization.

Pawaria S, Binder RJ
Nature communications 2011 Nov 1;2:521

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Identification of the receptor scavenging hemopexin-heme complexes.

Hvidberg V, Maniecki MB, Jacobsen C, Højrup P, Møller HJ, Moestrup SK
Blood 2005 Oct 1;106(7):2572-9

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All for CD91 and CD91 for all.

Stebbing J, Savage P, Patterson S, Gazzard B
The Journal of antimicrobial chemotherapy 2004 Jan;53(1):1-3

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CD91 is a common receptor for heat shock proteins gp96, hsp90, hsp70, and calreticulin.

Basu S, Binder RJ, Ramalingam T, Srivastava PK
Immunity 2001 Mar;14(3):303-13

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Prolow-density lipoprotein receptor-related protein 1 was performed using 70% confluent log phase U-87 MG and SK-BR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD91 Monoclonal Antibody (A2MR-a2), eFluor 660, eBioscience™ (Product # 50-0919-41, 50-0919-42) at 1ug/ml in 0.1% BSA, incubated at 4 degree celsius overnight ..(Panel a: Red). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Green) was stained with Alexa Fluor™ 488 Phalloidin (Product # A12379, 1:300 dilution). Panel d represents the merged image showing golgi localization. Panel e represents SK-BR-3 cells showing no expression of Prolow-density lipoprotein receptor-related protein 1.Panel f represents control cells with isotype control antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (blue histogram) or Anti-Human CD91 eFluor® 660 (purple histogram). Cells in the monocyte gate were used for analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Fibrogenic differentiation of human embryonic stem cell-derived mesenchymal stem cells (hESC-MSCs) upon stimulation with connective tissue growth factor (CTGF). hESC-MSCs were differentiated into fibroblasts by treatment with various concentrations of connective tissue growth factor (CTGF) for 4 weeks. Normal skin fibroblasts (Detroit 551) were also used as a positive control. (a) mRNA levels of fibroblast-related genes in hESC-MSCs after CTGF treatment were determined by the real-time polymerase chain reaction (PCR) ( n = 3, one-way ANOVA; ** p < 0.01 and **** p < 0.0001). (b) Collagen (Col)1, Col3, fibronectin (FN), and fibroblast-specific protein- (FSP-) 1 mRNA levels were determined by PCR. (c) FN, FSP-1, Col1, and beta -actin protein levels in hESC-MSCs following CTGF treatment were determined by immunoblotting. (d) Masson's trichrome was used to detect collagen fibers. (e) hESC-MSCs were immunostained to detect collagen I (Col1) following CTGF treatment. 4',6'-Diamidino-2-phenylindole (DAPI) was used for nuclear counterstaining. (f) Flow cytometry analysis of hESC-MSCs. After expansion of hESC-MSCs and hESC-MSC-Fbs, cells were trypsinized and stained with specific markers for CD29, CD47, CD73, CD90, CD91, CD105, and CD166.