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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-47784 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SULT2A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) SULT1A1, rhSULT1A3, rhSULT1B1, rhSULT1E1, and rhSULT4A1 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of human Cytosolic Sulfotransferase 2A1/SULT2A1 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47784) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Cytosolic Sulfotransferase 2A1/SULT2A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SULT2A1 in human liver tissue. Samples were incubated in SULT2A1 polyclonal antibody (Product # PA5-47784) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti‚Sheep IgG secondary antibody. A specific band was detected for Cytosolic Sulfotransferase 2A1/SULT2A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of SULT2A1 in immersion fixed HepG2 human hepatocellular carcinoma cell line. Samples were incubated in SULT2A1 polyclonal antibody (Product # PA5-47784) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
