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LearnNBP2-33953
antibody from Novus Biologicals
Targeting: MCM2
BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [8]
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- Product number
- NBP2-33953 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal MCM2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human MCM2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM2 Antibody [NBP2-33953] - Analysis in control (vector only transfected HEK293T lysate) and MCM2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM2 Antibody [NBP2-33953] - Analysis in human cell line HEL.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human skin shows strong nuclear positivity in basal layer epidermal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human kidney shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human lymph node shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Analysis in human lymph node and liver tissues. Corresponding MCM2 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human liver shows no positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human pancreas shows low positivity in exocrine glanular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCM2 Antibody [NBP2-33953] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
