LS-C330979

antibody from LSBio

Targeting: MCM2 BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C330979

Provider

LSBio

Product name

MCM2 Antibody LS-C330979

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of extracts of HeLa cells.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using MCM2 antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of extracts of HeLa cells.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using MCM2 antibody at 1:500 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse spleen using MCM2 antibody(40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using MCM2 antibody(40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human esophageal cancer using MCM2 antibody(40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human stomach using MCM2 antibody(40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human esophageal cancer using MCM2 antibody(40x lens).